Plastic Biodegradation by Peseudomonas aeruginosa UKMCC1011 Using A Modified Winogradsky Column

Nelvi Damayanti, Norela Sulaiman, Nazlina Ibrahim

Abstract


The use of plastic as a packaging has been increased in the market. Plastics was polymers that difficult to degrade in nature which to be a source of environmental damage. This study was conducted to see the potential of Pseudomonas aeruginosa UKM1011 and natural bacteria to degrade plastics using modified Winogradsky columns. The natural substrate used as soil, sand, and water were taken from Tasik Kejuruteraan UKM Bangi, Selangor. Modified Winogradsky columns using 600ml mineral bottles filled with 300 gr of soil or sand as a substrate and 300 ml of water mixed with medium salt as nutrients. The degradation process carried out for 60 days with parameters were the measurement of optical density (OD) at 600nm, pH conditions, and percent weight loss every 15 days. The results show P. aeruginosa were able to utilize plastics as the carbon source in modified Winogradsky column. The highest plastic degradation was black plastic on the sand substrate by the added P. Aeruginosa was 1.6684% followed by OD600was 0.599 and pH 8.2. While the high test degradation of yellow plastic on the sand substrate was 1.2302% followed by OD600was 0.593 and pH 7.9.

Keywords


Biodegradation, Plastic, Pseudomonas aeruginosa, Winogradsky

Full Text:

PDF

References


Marnika Sharma, Pratibha Sharma, Anima Sharma & Subhash Chandra. 2015. Microbial Degradation of Plastic – A Brief Review. CIBTech Journal of Microbiology 4(1): 85-89.

Kaseem, M., Hamad, K. & Deri, F. 2012. Thermoplastic Starch Blends. A Review of Recent Works 54: 165-176.

Ainiyah, D.N., & Shovitri, M. 2014. Bakteri Tanah Sampah Pendegradasi Plastik dalam Kolom Winogradsky. Jurnal Sains dan Seni ITS 3(2): 63-66.

Badriyah, L., & Sovitri, M. 2015. Biodegradasi Plastik Putih dalam Kolom Winogradsky. Jurnal Sains dan Seni ITS 4(2): 50-54.

Fadlilah, F.R., & Sovitri, M. 2014. Potensi Isolat Bakter iBacillus dalam Mendegradasi Plastik dengan Metode Kolom Winogradsky. Jurnal Teknik POMITS 3(2): 40-43.

Gu, J.D. 2000. Microbial Corrosion of Metals: The Uhlig Corrosion H&book. 2nd edition. Ed. Mitton, D.B & Mithchel, R. New York: Willey

Muller, R.J. 2001. Biological degradation of synthetic polyesters–enzymes as potential catalyst for polyester recycling. Process Biochemistry 41: 2124-2128.

Saraswati, R., Husen, E. & Simanungkalit, R.D.M. 2007. Metode Analisis Biologi Tanah. Jawa Barat: Balai Besar Penelitian dan Pengembangan Sumber Daya Lahan Pertanian.

Tortora, G.J., Funke, B.R., & Case, C.L. 2010. Microbiology an introduction. 10th Ed. San Francisco: Benjamin Cummings.

Alex, S. 2011. New perspective in plastic biodegradation. Journal of Current Opinion in Biotechnology 22: 422-426.

Paassen, L.A.V., Daza, C.M., Staal, M., Sorokin, D.Y., Van der Zon, W., Loosdrecht, V. 2016. Potential Soil Reinforcement by biological denitrification. Ecological Engineering 36: 168-175.

Maiti, C.K., Sen, S., Paul, A.K., Archarya, K., 2012. Pseudomonas aeruginosa WS-1 for biological control of leaf blight disease of Withania Somnifera. Plant Protect 45: 796-805.

Nakazawa, T., Furukawa, K., Haas, D., & Silver, S. 1996. Molecular Biology of Pseudomonas. Washington D.C: ASM PRESS.

Pelczar, M. J., & Chan, E. C. S. 2006. Dasar-Dasar Mikrobiologi. Jakarta: UI Press.

Lehninger, A.L. 1982. Dasar-Dasar Biokimia Jilid 1. Trans. Maggy Thenawijaya. Jakarta: Erlangga.

Zheng, Y., Yanful, E.K., Bassi, A.S. 2005. A Review of Plastic Waste Biodegradation. Critical Rev Biotechnology 25: 243-250.

Orhan, Y., Hrenovic, J. &Buyukgungor, H. 2004. Biodegradation of Plastic Compost Bags under Controlled Soil Conditions. Acta Chim 51: 579-588.

Willey, J.M., Sherwood., & Woolverton. 2008. Prescott, Harley, & Klein's Microbiology Seventh Edition. New York: McGraw-Hill Companies.

Sangale, M.K., Shahnawaz, M., Ade, A.B. 2012. A review on biodegradation of Polythene: The microbial approach. Journal Bioremed Biodegradation 3(10):164-172.

Sara Fatima. 2014. Chemical recycle of plastic. American Journal Engineering Research 3(11): 93-108.

Raziya fathima, M., Praseetha, P.K. & Rimal Isaac, R.S. 2016. Microbial degradation of Plastic Waste: A review. Journal of pharmaceutical, Chemical and Biological 4(2): 231-242.

Zavarzin, G.A. 2006. Winodgradsky a modern microbiology. Microbiology 75(5): 501-511.

Singh, B & Sharma, N. 2008. Mechanistic implementation of plastic degradation. Polymer Degradation and Stability 93: 561-584.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

e-ISSN No: 2356-2536
p-ISSN No: 2528-6854

Supported by :


Jurnal Online
Management Jurnal Elektronik


Pusat Dokumentasi dan Informasi Ilmiah
Lembaga Ilmu Pengetahuan Indonesia



Indexed by :